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usingmonoclonal anti ubiquitin  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology usingmonoclonal anti ubiquitin
    Usingmonoclonal Anti Ubiquitin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 5372 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/usingmonoclonal+anti+ubiquitin/Ubiquitin+Antibody/pm23810531-106-8-10
    Average 96 stars, based on 5372 article reviews
    usingmonoclonal anti ubiquitin - by Bioz Stars, 2026-09
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    Western Blot:

    Article Title: VAL- and AtBMI1-mediated H2Aub initiate the switch from embryonic to postgerminative growth in Arabidopsis.
    Article Snippet: Pull-down and coIP western blots were proved with monoclonal anti-FLAG M2 (Sigma-Aldrich F1804) and polyclonal anti-HA (Sigma-Aldrich H6908) antibodies, respectively. .. Western blot analysis of histone extracts was performed usingmonoclonal anti-ubiquitin (Santa CruzBiotechnology sc-8017) or anti-hH2Aub (ubiquityl-H2AK119 XP rabbit monoclonal antibody D27C4; Cell Signaling Technology 8240) antibodies. .. ChIP assayswere carried out on fixed chromatin extracted from seedlings at 10 DAG using polyclonal anti-trimethyl H3K27 (Diagenode pAb-069-050) or anti-hH2Aub (ubiquityl-H2AK119 XP rabbit monoclonal antibody D27C4; Cell Signaling Technology 8240) antibodies.



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    usingmonoclonal anti ubiquitin  (Santa Cruz Biotechnology)
    96
    Santa Cruz Biotechnology usingmonoclonal anti ubiquitin
    Usingmonoclonal Anti Ubiquitin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/usingmonoclonal+anti+ubiquitin/Ubiquitin+Antibody/pm23810531-106-8-10
    Average 96 stars, based on 1 article reviews
    usingmonoclonal anti ubiquitin - by Bioz Stars, 2026-09
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    usingmonoclonal antibody p4d1  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology usingmonoclonal antibody p4d1
    FIGURE 5. Dislocation of MHC class I-ubiquitin fusion proteins by US11. A, TS20 and TS20-US11 cells expressing UbK3R-A2K3R or UbWT-A2K3R fusion proteins were pulse-labeled for 10 min with [35S]Met/[35S]Cys and chased for 30 and 90 min at 33 °C with or without the proteasome inhibitor ZL3H. HLA-A2 molecules with (CHO) or without (CHO) carbohydrate side chain were immunoprecipitated with the MR24 anti-heavy chain serum and analyzed on an SDS-polyacrylamide gel (10%). The t 0 samples were treated with N-gly- canase F (endoF). B, experiment as in panel A, using cells expressing UbK3R-A2WT or UbWT-A2WT fusion proteins. C, A2WT and A2K3R constructs were translated in vitro in the presence of [35S]methionine in the absence of microsomes. One-third of the translation product was loaded on gel directly (left panel). Two-thirds of the translation product were subjected to immunoprecipitation with anti heavy chain antibody (MR24). One-half of the immunoprecipitate was loaded on gel (middle panel), and the other half was subjected to a subsequent reimmunoprecipitation with the monoclonal antibody <t>P4D1,</t> recognizing polyubiquitin chains (right panel). D, UbK3R-A2K3RandUbWT-A2K3Rconstructsweretranslatedinvitroandloadedontoa10%SDS-polyacrylamide gel.
    Usingmonoclonal Antibody P4d1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/usingmonoclonal+anti+ubiquitin/Ubiquitin/10__1074_slash_jbc__m602248200-78-3-6
    Average 96 stars, based on 1 article reviews
    usingmonoclonal antibody p4d1 - by Bioz Stars, 2026-09
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    FIGURE 5. Dislocation of MHC class I-ubiquitin fusion proteins by US11. A, TS20 and TS20-US11 cells expressing UbK3R-A2K3R or UbWT-A2K3R fusion proteins were pulse-labeled for 10 min with [35S]Met/[35S]Cys and chased for 30 and 90 min at 33 °C with or without the proteasome inhibitor ZL3H. HLA-A2 molecules with (CHO) or without (CHO) carbohydrate side chain were immunoprecipitated with the MR24 anti-heavy chain serum and analyzed on an SDS-polyacrylamide gel (10%). The t 0 samples were treated with N-gly- canase F (endoF). B, experiment as in panel A, using cells expressing UbK3R-A2WT or UbWT-A2WT fusion proteins. C, A2WT and A2K3R constructs were translated in vitro in the presence of [35S]methionine in the absence of microsomes. One-third of the translation product was loaded on gel directly (left panel). Two-thirds of the translation product were subjected to immunoprecipitation with anti heavy chain antibody (MR24). One-half of the immunoprecipitate was loaded on gel (middle panel), and the other half was subjected to a subsequent reimmunoprecipitation with the monoclonal antibody P4D1, recognizing polyubiquitin chains (right panel). D, UbK3R-A2K3RandUbWT-A2K3Rconstructsweretranslatedinvitroandloadedontoa10%SDS-polyacrylamide gel.

    Journal: Journal of Biological Chemistry

    Article Title: Ubiquitination of MHC Class I Heavy Chains Is Essential for Dislocation by Human Cytomegalovirus-encoded US2 but Not US11

    doi: 10.1074/jbc.m602248200

    Figure Lengend Snippet: FIGURE 5. Dislocation of MHC class I-ubiquitin fusion proteins by US11. A, TS20 and TS20-US11 cells expressing UbK3R-A2K3R or UbWT-A2K3R fusion proteins were pulse-labeled for 10 min with [35S]Met/[35S]Cys and chased for 30 and 90 min at 33 °C with or without the proteasome inhibitor ZL3H. HLA-A2 molecules with (CHO) or without (CHO) carbohydrate side chain were immunoprecipitated with the MR24 anti-heavy chain serum and analyzed on an SDS-polyacrylamide gel (10%). The t 0 samples were treated with N-gly- canase F (endoF). B, experiment as in panel A, using cells expressing UbK3R-A2WT or UbWT-A2WT fusion proteins. C, A2WT and A2K3R constructs were translated in vitro in the presence of [35S]methionine in the absence of microsomes. One-third of the translation product was loaded on gel directly (left panel). Two-thirds of the translation product were subjected to immunoprecipitation with anti heavy chain antibody (MR24). One-half of the immunoprecipitate was loaded on gel (middle panel), and the other half was subjected to a subsequent reimmunoprecipitation with the monoclonal antibody P4D1, recognizing polyubiquitin chains (right panel). D, UbK3R-A2K3RandUbWT-A2K3Rconstructsweretranslatedinvitroandloadedontoa10%SDS-polyacrylamide gel.

    Article Snippet: Ubiquitin was detected usingmonoclonal antibody P4D1 (SantaCruz Biotechnology).

    Techniques: Ubiquitin Proteomics, Expressing, Labeling, Immunoprecipitation, Construct, In Vitro